The Absorbency Spectrum and Photosensitivity of a New Synthetic Visual Pigment Based on 4-Hydroxyretinal

Publication Type:Journal Article
Year of Publication:1992
Authors:Y. Kito, Partridge, J. C., Seidou, M., Narita, K., Hamanaka, T., Michinomae, M., Sekiya, N., Yoshihara, K.
Journal:Vision Research
Date Published:Jan
Type of Article:Article
ISBN Number:0042-6989
Accession Number:ISI:A1992GY49400002
Keywords:4-HYDROXYRETINAL, ANALOG, BIOLUMINESCENT SQUID, chromophore, eye, membrane, META-RHODOPSIN, retinal, RETINALS, rhodopsin, visual pigment, WATASENIA-SCINTILLANS

The firefly squid, Watasenia scintillans, is the only animal known to possess a visual pigment in which the chromophore is 4-hydroxyretinal. This paper describes the absorbance spectrum and some properties of a synthetic "A4" visual pigment generated from bovine opsin and 4-hydroxyretinal. The absorbance spectrum of this pigment is compared with (a) bovine rhodopsin and (b) a rhodopsin template with the same lambda-max as the synthetic visual pigment. The A4 pigment is shown to have an absorbance spectrum that is almost identical to that of a rhodopsin template. It is also shown that the photosensitivity and thermal stability of the A4 pigment, dispersed in detergent micelles, is essentially similar to that of rhodopsin.

Alternate Journal:Vision Res.
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