|Publication Type:||Journal Article|
|Year of Publication:||2010|
|Authors:||C. A. Hermosilla, Rocha, F., Fiorito, G., Gonzalez, A. F., Guerra, A.|
|Journal:||Ices Journal of Marine Science|
Daily periodicity of growth increments in stylets was validated in wild-caught Octopus vulgaris maintained under controlled conditions. It was corroborated by staining the stylets either with oxytetracycline (OTC) or tetracycline (TC), and comparing the number of rings produced with the number of days elapsed. In all, 19 animals (10 males and 9 females; 680-1470 g body weight, BW) were injected with OTC in Vigo (mean 124 mg kg(-1)), and another six animals (1 male and 5 females; 248-570 g BW) with TC at Naples (mean 120 mg kg 21). The animals were successfully maintained in captivity until sacrificed for up to 6 (one animal), 9 (one animal), 18 (the six animals from Naples), and 21 (17 animals) days. The number of increments counted in transverse stylet sections was 18.9 +/- 1.4 and 20.5 +/- 1.5 for octopuses maintained for 18 and 21 d, respectively. The mean rate of increment formation was 1.02 increments per day, suggesting a periodicity of 1 increment per day in the stylet. Consequently, the results successfully validate daily increment deposition in O. vulgaris stylets in the size range analysed.